Specific detection of nucleotides creatine phosphate and their derivatives from tissue samples in a simple isocratic recycling low volume system
Summary
Type: HPLC Application #: 107270
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: photodiode array Conditions: eluent: degassed 25 mM potassium phosphate buffer with 2 mmol/l 11-aminoundecanoic acid (C11AA) in water - methanol (92:8, v/v) temperature 20 °C flow rate 1.0 ml/min Authors: Fischer JH Source: LC-GC int 8 (1995) 254
Details
Legend: Fig. 3a. Chromatogram of a standard mixture of several tri-, di- and
mononucleotides at a pH of 6.4, registration at 210 nm. Peaks: A = creatine, B
= creatine phosphate, C = cytidine 5'-diphosphate